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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Tonic ATP-mediated growth suppression in peripheral nerve glia requires arrestin-PP2 and is evaded in NF1

Fig. 3

ATP-P2Y2 suppresses SC proliferation by modulating PP2A activity. (a) WT mSC were treated with non-hydrolysable purine analogues; ATPγS had the most significant effect on SC growth (p < 0.0001). (b) iHSC growth was suppressed by ATPγS and rescued by the P2Y2 antagonist AR-C 118925XX (AR-C). (c) WT mSC growth suppression by ATPγS was rescued by AR-C. (d) Western blot confirms reduced ARRB1 and ARRB2 protein by shRNA knockdown. (e) iHSCs treated with shRNA ARRB1 and 2 shown reduced growth suppression in response to ATPγS. (f) Western blots of WT mSCs lysates after ATPγS treatment show increases in pERK 1/2 and pSer473 Akt. A decrease in pThr308 AKT was observed by 40 min

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