Skip to main content
Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Endothelial damage, vascular bagging and remodeling of the microvascular bed in human microangiopathy with deep white matter lesions

Fig. 8

Parenchymal and perivascular CD68-positive macrophages and IBA1-positive microglial cells in white matter areas in thick sections double-labeled for COLL4 and UEA-l, respectively. 11a-b In NoSVD controls, IBA1- and CD68-positive cellular processes are observed in the subcortical white matter, but the density of labeled cell bodies is rather low compared to SVD cases. c-d In “pure” SVD, more IBA1- and CD68-positive cells with thicker processes are found in the white matter parenchyma compared to NoSVD. Some CD68 positive cells have a close relationship to the wall of vessels. Many IBA1-labeled cells have complex morphologies and clusters with IBA1-labeled cells somata are seen. e-h In cases with SVD + VBI, DWMLs exhibit numerous enlarged CD68-positive macrophages with a phagocytic phenotype, and COLL4-positive dots are found among CD68-positive cells (thin arrow in inset of g). The density of IBA1-positive microglial cells is also high in the DWMLs, and their morphology indicates an activated (hypertrophic) or a phagocytic (round) state. Thick arrows (insets in c and g) show examples of CD68-positive cells quantified at perivascular sites with a direct association to the wall of COLL4-positive vessels. Images were obtained from Case 2 (ovarian cancer, NoSVD) (a-b), Case 5 (pulmonary embolism, pure SVD) (c-d), and Case 12 (pontine bleeding, SVD + VBI) (e-h). For a more detailed morphology of IBA1-positive cells, also see Additional file 3: Video S2 of Microglia. Scale bars: 100 μm (a, c, e and g) and 50 μm (b, d, f and h)

Back to article page