Fig. 1
MATR3 is overexpressed in the muscle of MATR3WT and MATR3F115C transgenic mice. a Northern blot analysis of quadriceps showed transgenic MATR3 RNA levels. Endogenous murine PrP mRNA, which is recognized by the same probe used to detect transgenic mRNA, was utilized as a loading control. b Quantification of Northern blots confirmed significant increases in MATR3 levels from lines 1563, 1554, and 1576. MATR3 values were normalized to PrP. One-way ANOVA, p = 0.0308. Bonferroni’s multiple comparison test showed that lines 1576 and 1554 were significantly different (p < 0.05), while there was no difference between lines 1576 to 1563 and 1554 to 1563. c Representative western blot of gastrocnemius using an antibody that recognizes both human and murine MATR3 showed an increase in total MATR3 (120 doublet, 90, 75, and 55 kDa combined) in ~ 2 month old transgenic mice from MATR3WT lead line 1563 and MATR3F115C lead line 1576 compared to NT mice. d Quantification of western blot confirmed significant increase in total MATR3 levels (120 doublet, 90, 75, and 55 kDa combined) from MATR3WT and MATR3F115C lines compared to NT. One-way ANOVA, p ≤ 0.0001. Bonferroni’s multiple comparison test showed that total MATR3 levels from lines 1563 (p ≤ 0.05) and 1576 (p ≤ 0.0001) were statistically significant from NT. MATR3 levels from lead line 1576 were significantly elevated compared to 1563 (p ≤ 0.0001). e Western blot analysis of gastrocnemius showed an increase in MATR3 levels in ~ 10 month old transgenic mice from MATR3F115C lead line 1576 compared to NT. f Quantification of western blot confirming significant increase in total MATR3 levels (120 doublet, 90, 75, and 55 kDa combined) from MATR3F115C mice compared to NT mice. One-way ANOVA, p ≤ 0.01. Bonferroni’s multiple comparison test showed that line 1576 (p ≤ 0.0001) was statistically significant from NT. There is no significant difference between lead line 1576 compared to lead line 1563, as well as lead line 1563 compared to NT (p > 0.05). *, p ≤ 0.05; **, p ≤ 0.01, ****, p ≤ 0.0001. Arrow head indicates expected MATR3 band at 120 kDa, arrows indicate lower molecular weight species as indicated