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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Mutant UBQLN2P497H in motor neurons leads to ALS-like phenotypes and defective autophagy in rats

Fig. 7

Expression of mutant UBQLN2P497H in the astrocytes of rats. a A graph of the GFAPtTA construct. The trasgene is regulated by DOX. b-c Western blots show the relative expression level of human UBQLN2 in bigenic GFAPtTA/UBQLN2P497H (P497H: TG) but not in control rats (GFAPtTA: CT) at 1 month old. rUB2: endogenous UBQLN2, hUB2: human UBQLN2, and the * indicates unknown bands. The data are reported as the mean ± standard deviation (n = 4, female rats were used). d-e The projection of confocal images shows the expression of human UBQLN2P497H in the astrocytes of spinal cords in P497H but not in GFAPtTA rats. At 6 months old, a substantial proportion of UBQLN2 inclusions are mislocalized into the nuclei of astrocytes in P497H rats (e). Scale bars: 20 μm

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