Fig. 2

Soluble Aβ extracts that either do or do not block LTP affect human neurites accordingly. IncuCyte live-cell video microscopy monitored the effect of AD brain extracts on iPSC-derived neurogenin-induced human neurons (iNs). On post-induction day 21, iNs were treated with Control TBS (Control: black) or AD extracts (colors) and the neurons imaged for 72 h. (a) Phase contrast images (top panel) at 0 and 72 h were analyzed using the incuCyte NeuronTrack algorithm to identify neurites (pseudocolored pink in middle panels). Identified neurites were superimposed on the phase contrast image (bottom panel). Scale bar, 100 μm. Each well of iNs was imaged for 6 h prior to addition of the extract and NeuroTrack measured neurite length and branch points at this baseline used to normalize neurite length measured at each interval after addition of extract. (b,c) Time course of change in neurite length (b) and branch points (c) after addition of AD brain extracts (red and blue) or immunodepleted sample (light brown and light blue, respectively) when compare to control brain extract (black). Summary results at 72 h treatments are shown on right