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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Satellite cells maintain regenerative capacity but fail to repair disease-associated muscle damage in mice with Pompe disease

Fig. 6

Gaa−/− satellite cells regenerate muscle and self-renew after serial injury. a. Experimental schedule. Black arrows indicate the time at which TA muscles were collected for analysis, red arrows indicate the time of injury. b. HE staining of TA sections before and 3 weeks after the third injury. Representative images are shown. c. Quantification of fiber diameter in WT (left) and Gaa−/− (right) TA muscle after serial injury from (b). d. Quantification of the number of Pax7-positive cells by immunofluorescent staining of TA sections using a Pax7 antibody in WT (left) and Gaa−/− (right) TA muscle after serial injury. e. Quantification of the number of Pax7/Ki67 double-positive cells by immunofluorescent staining of TA sections using Pax7 and Ki67 antibodies in WT (left) and Gaa−/− (right) TA muscle after serial injury. Data in c-e are means ± SD from at least 2 muscles derived from 2 different animals. For comparison with levels of indicated parameter in uninjured age-matched TA muscles, values at 40 weeks of age (to compare with the start of the experiment) and at 60 weeks of age (to compare with the end of the experiment) are included in (c-e)

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