Fig. 4

Gaa^−/−mice regenerate muscle efficiently after experimental injury. a. Schematic representation of the injury experiment. Black arrows indicate the time at which TA muscles were collected for analysis, the red arrow indicates the time of injury. b. HE staining of TA sections before (Uninjured, 0 days post injury (DPI)) and at 15 days DPI with BaCl₂ at three ages. Representative images are shown. c. Quantification of fiber diameter from (b). d. Schematic representation of injury experiment with a longer follow up after injury. Black arrows indicate the time at which TA muscles were collected for analysis, red arrow indicate the time of injury. e. HE staining of TA sections of the injury experiment with long follow-up. Representative images are shown. f. Quantification of fiber diameter from E. Data in C and F are means ± SD from at least 3 muscles derived from 2 or more different animals. *p < 0.05; **p < 0.01 and ***p < 0.001