Fig. 2

Gaa^−/− mice display modest and transient muscle regeneration during disease progression. a. eMyHC expression. Immunofluorescent staining of TA sections using a MyHC antibody (in red). Representative images are shown. The basal lamina was stained using a Laminin antibody (in green). Nuclei were stained with Hoechst (in blue). Black and white images of eMyHC staining are included for better visualization. b. Central nucleated fibers. Representative images of TA sections stained with Laminin (in red) and Hoechst (in white). c. Quantification of central nucleated fibers from B. Data represent means ± SD (n = 2–3 muscles from at least 2 different animals per genotype per timepoint). *p < 0.05. **p < 0.01 and ***p < 0.001