Fig. 5
From: Formation of Toxic Oligomeric Assemblies of RNA-binding Protein: Musashi in Alzheimer’s disease
Neuronal MSI2 oligomers in AD Brain (a) Representative epifluorescence images of AD cortical section showing co-localization between α-MSI2 (red), NeuN (green) and α-Oligomer antibody (F11G3, blue) (white scale bar: 10 μm, magnification: 40X). (b) Twice zoomed image of ROI (green dashed line) highlighting localization of MSI2 oligomers in neurons (white scale bar: 5 μm, magnification 40X). (c) PCC of 5 ROIs in AD cortex section represented in bar graphs showing the levels of co-localization between MSI2 and F11G3 (PCC: 0.9768 ± 0.01763), MSI2 and NeuN (PCC: 0.7581 ± 0.1237) and F11G3 and NeuN (PCC: 0.8004 ± 0.1149) signals. (d) LUT Fire image of selected ROI between MSI2 and α-Oligomer antibody (F11G3) confirming the presence of MSI2 oligomers in neurons with heterogeneous distribution in the cytoplasm (color scale bar represents pixel intensity)