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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Transcriptomic and epigenetic profiling of ‘diffuse midline gliomas, H3 K27M-mutant’ discriminate two subgroups based on the type of histone H3 mutated and not supratentorial or infratentorial location

Fig. 4

Distribution of H3K27me3 epigenetic marks in in vitro models of DIPG. a Genome distribution of H3K27me3 peaks in H3.1- and H3.3-K27M GSC cells. Pie-charts represent the genomic annotation of the genomic loci bound by H3.3K27me3 in each subgroup performed using ChIPseeker package. The majority of the H3K27me3 occupied regions are located within the distal intergenic regions, and a small number of peaks are located in upstream regions. The percentage of each feature in H3.1- and H3.3-K27M subgroups is indicated in the legend. b-c H3K27me3 levels in overlapping (b) or non-overlapping (c) gene regions of 10 kb were normalized to equivalent total number of tags in the samples (in columns), and genomic intervals were subsequently clustered by k-means (k = 5). Left and right borders represent − 5 kb and + 5 kb, respectively. Blue color scale bar indicates relative coverage. Average signal of all H3K27me3 peak regions of each cluster is presented at the top. The number of genomic loci in each cluster is indicated in the legend

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