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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Transcriptomic and epigenetic profiling of ‘diffuse midline gliomas, H3 K27M-mutant’ discriminate two subgroups based on the type of histone H3 mutated and not supratentorial or infratentorial location

Fig. 2

Classification of high-grade gliomas based on genome-wide DNA methylation profiles. a t-SNE analysis of the methylation profiles of 80 pediatric high-grade gliomas using the topmost differentially methylated probes across the sample set (s.d. > 0.25). Midline tumors are color-coded according to the histone H3 gene mutated: dark green for H3.1-K27M (n = 13), purple for H3.2-K27M (n = 1) and light green for H3.3-K27M tumors (n = 36). Others H3-WT high-grade glioma are also presented: H3.3-G34R mutated tumors (n = 10, blue), PDGFRA (n = 10, orange) and MYCN (n = 10, brown) amplified tumors. b-c Analysis of methylation patterns of 50 pediatric H3-K27M midline tumors by t-SNE indicates that H3.1-K27M and H3.3-K27M tumors are clearly distinct from each other. Dimensionality reduction and visualization of methylome data was performed by t-SNE after selection of the probes with the greatest variance (n = 10,000; See Methods). Samples were color-coded according to their location (b), the histone H3 gene mutated (c). t-SNE show two main clusters corresponding to H3.1/H3.2-K27M and H3.3-K27M subgrouping

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