Fig. 2

CT100(I716F) overexpression does not affect morphology of granule cells in adult mice. a Examples of traced DG granule cells after biocytin filling. b Sholl analysis shows that neither CT100(I716F)-overexpression nor GluN1 knockout affects the number of intersections of granule cell dendrites. There is no difference in the total dendritic length of neurons in the different groups. c Examples of maximum intensity projections of z-stacks of GluN2B^fl/fl, GluN2B^fl/fl/CT100(I716F), GluN2B^−/− and GluN2B^−/−/CT100(I716F) granule cell dendrites. d + e + f Spine number is not affected after three weeks of CT100(I716F)-overexpression. Spine number is reduced in GluN1^−/− (d), and GluN2B^−/− (f) granule cells. g + i + j The distribution of stubby, thin and mushroom spines is slightly affected by CT100(I716F)-overexpression and/or NMDAR subunit knockout with fewer thin spines in GluN2B^fl/fl vs GluN2B^−/−; GluN2B^−/− vs GluN2B^−/−/CT100(I716F) and an increase in stubby spines in GluN2A^−/− vs GluN2A^−/−/CT100(I716F); GluN2B^fl/fl vs GluN2B^fl/fl/CT100(I716F); GluN2B^fl/fl vs GluN2B^−/−. Bar graphs show median ± IQR. * = p < 0.05, ** = p < 0.01, *** = p < 0.001; morph. = morphology