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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: NMDA receptors mediate synaptic depression, but not spine loss in the dentate gyrus of adult amyloid Beta (Aβ) overexpressing mice

Fig. 2

CT100(I716F) overexpression does not affect morphology of granule cells in adult mice. a Examples of traced DG granule cells after biocytin filling. b Sholl analysis shows that neither CT100(I716F)-overexpression nor GluN1 knockout affects the number of intersections of granule cell dendrites. There is no difference in the total dendritic length of neurons in the different groups. c Examples of maximum intensity projections of z-stacks of GluN2Bfl/fl, GluN2Bfl/fl/CT100(I716F), GluN2B−/− and GluN2B−/−/CT100(I716F) granule cell dendrites. d + e + f Spine number is not affected after three weeks of CT100(I716F)-overexpression. Spine number is reduced in GluN1−/− (d), and GluN2B−/− (f) granule cells. g + i + j The distribution of stubby, thin and mushroom spines is slightly affected by CT100(I716F)-overexpression and/or NMDAR subunit knockout with fewer thin spines in GluN2Bfl/fl vs GluN2B−/−; GluN2B−/− vs GluN2B−/−/CT100(I716F) and an increase in stubby spines in GluN2A−/− vs GluN2A−/−/CT100(I716F); GluN2Bfl/fl vs GluN2Bfl/fl/CT100(I716F); GluN2Bfl/fl vs GluN2B−/−. Bar graphs show median ± IQR. * = p < 0.05, ** = p < 0.01, *** = p < 0.001; morph. = morphology

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