Fig. 5

Lipid accumulation in muscle fibers from Gaa^−/− and WT mice using infrared (IR) microspectroscopy. a: Representative raw IR spectra obtained from Tibialis anterior (TA) muscle fibers from Gaa^−/− and WT mice at 1.5 and 9 mo of age (spectral range: 3800–1400 cm^− 1). b, c: Surface area under the curve of the ester peak and the peak corresponding to the C-H linkage of lipids (spectral ranges: 1770–1720 cm^− 1 and 3100–2800 cm^− 1, respectively) normalized against the protein peak area (spectral range: 1720–1480 cm^− 1). Statistics: Two-way ANOVA with Sidak post hoc test; n = 3 animals per group; **p < 0.01