Fig. 4

Autophagic vacuole and enlarged lysosome accumulation in skeletal muscles from Gaa^−/− mice. a: Cross-sections of Tibialis anterior (TA) and Triceps brachii (TB) muscles from Gaa^−/− mice at 1.5, 4, 6 and 9 mo of age and 9-mo-old WT mice subjected to LC3 (green) and LAMP1 (red) immunolabeling. Nuclei are counterstained with DRAQ5 (blue). b: Longitudinal sections of TA and TB muscles from 9-mo-old Gaa^−/− mice following immunolabeling with anti-LC3 (green) and anti-LAMP1 (red) antibodies. Nuclei are counterstained with DRAQ5 (blue). Scale bars = 50 μm