Fig. 8

JAK2ca-induced astrocyte reactivity is sufficient to alter synaptic transmission and long-term plasticity in WT mice. a, Acute hippocampal slices were prepared from the hippocampus of 4–6 month-old WT-GFP and WT-JAK2ca mice. b-c, Representative paired-pulse stimulation traces for WT-GFP and WT-JAK2ca mice (100 ms interval). JAK2ca shifts the input/output relationship to the right, reducing the strength of basal glutamatergic transmission (b, Two way ANOVA and Bonferroni test) without impacting release probability, as revealed by unchanged PPR at 50 V (c, Student t test). N = 15–13. d, Representative (left) and average (right) fEPSPs before (1) and after (2) HFS protocol. LTP is impaired in WT-JAK2ca mice. e, Bar graph representing normalized fEPSP slopes 40 to 50 min post HFS. N = 8 in each group. Student t test. * p < 0.05, *** p < 0.001