Fig. 6

SOCS3 rescues synaptic transmission and long-term plasticity in the hippocampus of 3xTg mice. a, Acute hippocampal slices were prepared from the hippocampus of 8–9 month-old WT-GFP, 3xTg-GFP and 3xTg-SOCS3 mice. A recording electrode was placed in the stratum radiatum of the GFP⁺ CA1 region. b, Acute slices processed for GFAP immunohistochemistry (red). In 3xTg-GFP mice, astrocytes display higher GFAP immunoreactivity and tortuous processes, compared to WT-GFP controls. SOCS3 restores low GFAP levels in 3xTg astrocytes N = 8–7-6. c, Representative traces for WT-GFP, 3xTg-GFP and 3xTg-SOCS3 mice after a paired-pulse stimulation protocol (50 ms interval) with increasing voltage. The input/output relationship is impaired in 3xTg-GFP mice and restored by SOCS3. N = 11-7-7. Two way (group, voltage) ANOVA and Tukey’s test. d, The paired-pulse ratio (PPR) at 50 V is similar in the three groups. N = 11-7-7. ANOVA. e, Representative (left) and average (right) fEPSPs before (1) and after (2) HFS protocol in the three groups. LTP is impaired in 3xTg mice and restored by SOCS3. f, Normalized fEPSP slopes 40 to 50 min post HFS, relatively to fEPSPs measured 10 min before HFS. N = 6-6-5. ANOVA and Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.001