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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Alzheimer’s associated amyloid and tau deposition co-localizes with a homeostatic myelin repair pathway in two mouse models of post-stroke mixed dementia

Fig. 4

Stroke induces β-secretase (BACE) 1 and neuregulin (NRG) 1 type III expression in the white matter tracts of aged wildtype (wt) mice compared to young adult mice. a-c Western blotting with a BACE1 (~70 kDa) or NRG type III (~50 kDa) antibody detected a single band at the appropriate molecular weight in mouse brain lysates for each protein. Representative 20× images (n=3 mice/experimental group) of (a) BACE1+ and (b) NRG1 type III+ immunostaining (arrows) in the white matter tracts (thalamus-internal capsule) of 18 mo mice at 12 weeks after sham or stroke surgery (Equivalent = area imaged in wt-sham mice that is equivalent to the ipsilateral hemisphere imaged in wt-stroke mice; Contralateral = area imaged in the contralateral hemisphere of wt-stroke mice that is equivalent to the ipsilateral hemisphere of wt-stroke mice). Scale bar, 50 μm (BACE1 and NRG1 type III). Images revealed BACE1 and NRG1 type III expression in the ipsilateral white matter tracts of stroked, but not in sham-operated mice. c Representative 10× immunofluorescence images (n=3 mice/experimental group) of total tau+ immunostaining in the ipsilateral and contralateral white matter tracts of wt mice after stroke surgery. Scale bar, 125 μm. d Image: Representative 40× Fluoro-Jade staining in the ipsilateral white matter tracts of wt mice after stroke confirms that this is an area of axonal degeneration following DH stroke. Scale bar, 100 μm. Graph: Relative to naïve mice, there is significant Fluoro-Jade staining starting at 1 week (wk) post-stroke, continuing into at least 8 wk post-stroke. Data represent mean ± SEM from n=5 mice/experimental group. *p<0.05

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