Fig. 6

Increased PKC kinase activity in SCA14 patient cells. a Lysates of iPSCs were subjected to immunoblotting for PKCγ, T514- and T674-phosphorylated PKCγ and phospho-PKC substrates. Actin was used as loading control. b Quantification of PKCγ phosphorylation at T514 (upper panel) and T674 (lower panel) versus total PKCγ. Phosphorylation was significantly increased in SCA14 iPSCs compared to controls (n = 3, *p < 0.05, **p < 0.01, ANOVA followed by Bonferroni’s post-hoc test). c Lysates from post-mortem cerebellum were subjected to immunoblotting for PKCγ, T514-phosphorylated PKCγ, Calbindin and Actin. d Quantification of PKCγ phosphorylation at T514 versus total PKCγ. T514 phosphorylation was significantly increased in SCA14 (H101Q) cerebellum compared to controls (n = 3, **p < 0.01, unpaired students’ t-test). e Cerebellar lysates were subjected to immunoblotting for PKCγ, phospho-PKC substrates, phosphorylated (p) MARCKS, MARCKS and Actin