Preserving prion strain identity upon replication of prions in vitro using recombinant prion protein

Table 1 Serial transmission of rPrPres^{PE + PolyA} in Golden Syrian hamsters

Passage	n_sick/n_t ^a	n _PrP^Sc/n_t ^b	inc time ^c, dpi	duration ^d, days
1st	3/4	4/4	517	105
2nd ^e	6/6	6/6	274, 4 at 300, 324	110, 122, 126, 133, 134, 160
2nd ^e	5/5	5/5	274, 2 at 288, 324, 335	105, 116, 119, 146, 166

^a The number of animals that developed clinical signs per total number of animals
^b The number of animals with PrP^Sc on Western blot per total number of animals
^c Incubation time to first clinical signs
^d Duration of clinical disease from the first clinical signs to the terminal stage
^e For the second passage, two inocula were prepared using two individual animals from the first passage

ISSN: 2051-5960