Fig. 4
From: Preserving prion strain identity upon replication of prions in vitro using recombinant prion protein
Histopathological analysis of animals from the second passage of rPrPresPE + PolyA. PrPSc deposition in hippocampus (a), deep layers of cortex (e), thalamus (f), and cerebellum (g) as shown by 3F4 staining. Vacuolation and reactive gliosis were observed in the areas of PrPSc accumulation, as shown for hippocampus using staining with hematoxylin and eosine (c), or staining of microglia with anti-Iba1 (b) or astrocytes with anti-GFAP (d). Scale bars = 300 μm (a-d) or 200 μm (e-g)