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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Preserving prion strain identity upon replication of prions in vitro using recombinant prion protein

Fig. 1

Attempts to produce Ha-rPrPSc with assistance of PE and PolyA. a Duplicate sPMCA reactions were seeded with 103-fold diluted brain-derived Hyper (HY), Drowsy (DY) or SSLOW, or atypical PrPres produced in vitro, then subjected to four sPMCA rounds in the presence of PE with 3-fold dilutions between rounds and analyzed by Western blot. Products of 4th sPMCA rounds are shown. Small amounts of PK-resistant material (rPrPresPE) were detected in the reactions seeded with SSLOW (indicated by arrow). b sPMCA reactions were seeded with PMCA-derived rPrPresPE or 103-fold diluted SSLOW brain material, then subjected to four serial rounds in the presence of PE alone or a mixture of PE and polyA with 5-fold dilutions between rounds and analyzed by Western blots. c Serial amplification of rPrPresPE + PolyA in PMCA. sPMCA reactions were seeded with 103-fold diluted SSLOW brain material, then subjected to 18 serial rounds in the presence of PE and polyA with 5-fold dilutions between rounds and analyzed by Western blots. SAF-84 antibody was used to verify the absence of low molecular weight bands upon PK-digestion (middle panel)

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