Fig. 3

CMH suppresses endothelial VCAM-1 and ICAM-1 expression during EAE. a and c. Frozen sections of lumbar spinal cord taken from disease-free, EAE-normoxia or EAE-CMH mice at the peak symptomatic phase of EAE were stained for CD31 (AlexaFluor-488) and VCAM-1 (Cy-3) in panel A or CD31 (Cy-3) and ICAM-1 (AlexaFluor-488) in panel C. Scale bar = 100 μm (inset, scale bar = 50 μm). b and d. Quantification of VCAM-1 (b) and ICAM-1 expression (d). Results are expressed as the mean ± SEM (n = 6 mice/group). Note that CMH markedly suppressed vascular expression of VCAM-1 and ICAM-1. ** p < 0.01