Fig. 4
Extrinsic a-syn seeds were transmitted to the contralateral side within 24 h. a Mouse brains were stained with human a-syn-specific antibody LB509 (green) and anti-p-syn antibody (phospho S129) (red). Scale bars, 10 μm. b The number of human a-syn (left panels) and mouse a-syn (right panels) inclusions after human a-syn PFFs injection was quantified chronologically in each region of the brain: Str (Human a-syn p < 0.0001, mouse p-syn p < 0.0001), CTX (Human a-syn p < 0.0001, mouse p-syn p = 0.0008), EC (Human a-syn p < 0.0001, mouse p-syn p = 0.0014), and Amyg (Human a-syn p < 0.0001, mouse p-syn p < 0.0001). Data is represented as mean number of a-syn inclusions per region ± SEM, n = 5 mice per group, analysis of covariance (ANCOVA) was conducted to adjust area factor (ips, contra) to examine time related response. c Callosotomy 1 day before injection with human a-syn PFFs. d Callosotomy 1 day after injection with human a-syn PFFs. (c, d) The number of human a-syn (left panels) and mouse p-syn (right panels) inclusions (deposits) was quantified chronologically in each region (Str Human a-syn p = 0.0024, mouse p-syn p = 0.0114, CTX Human a-syn p = 0.0040, mouse p-syn p = 0.0484, EC Human a-syn p = 0.0216, mouse p-syn p = 0.0015, Amyg) of the brain. Horizontal axis: Time after human a-syn PFFs injection; Vertical axis: Number of a-syn inclusions/unit area (mm2). Data are the mean number of a-syn inclusions per region ± SEM, n = 5 mice per group, paired t-test for mouse and human a-syn at 3 weeks in CTX, Str, EC and Amyg for c and d. *p < 0.05,**p < 0.01,***p < 0.001, ****p < 0.0001. Str: striatum, CTX: cortex, EC: entorhinal cortex, Amyg: amygdala