Fig. 6

Tau phospho-immunoreactivity. Representative images of RZ3 (pThr231 tau) staining on JNPL3 brains. a, b, c Control mice received AAV5-eGFP injection (n = 15). Treated mice mice were injected with AAV5-CAG-scFvMC1 (n = 15) (d, e, f) or AAV5-GFAP-scFvMC1 (n = 15) (g, h, i) (bar: 500 μm a, d, g; bar: 100 μm b, c, e, f, h, i). j Quantification of percentage of area stained by RZ3 shows a significant reduction in the AAV5-GFAP-scFvMC1 injected group, in the CA1 region of hippocampus (**P = 0.0026 by one-way ANOVA) and (k) in the EC (**P = 0.0069 by one-way ANOVA). AAV5-CAG-scFvMC1 injected mice only showed a non-significant trend reduction in RZ3 staining in (j) CA1 (p = 0.0753) and in (k) EC (p = 0.1916). All graphs are means +/− SEM and were analyzed by one-way ANOVA, followed by Dunnett’s multiple comparison post hoc test, comparing every column to the eGFP injected group: **P < 0.01