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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Unique microglia recovery population revealed by single-cell RNAseq following neurodegeneration

Fig. 3

Emergence of a novel Apoe- and Ccl5-expressing microglial subset during recovery from neurodegeneration. a Heat map of four genes (Ccl5, Apoe, Cst3 and Sparc) that distinguish the tail cluster C9 (representing onset of recovery, 30 d) from C4 and C8 (representing both peak of disease at 7 d and onset of recovery at 30 d). Mean expression values are indicated. Microglia that upregulate Apoe and Ccl5 and down-regulate Cst3 and Sparc map specifically to the 30 d lesion group corresponding to onset of recovery. See Fig. 1d-e. The color legend represents log2(transcript counts). b-e tSNE representations highlighting the strong upregulation of b Ccl5 and c Apoe and downregulation of d Cst3 and e Sparc. Transcript counts across cells are shown as log2(transcript counts) in color legends. f-g Confocal images of a subset of lesion-specific FN microglia expressing f CCL5 or g APOE (green; filled arrowhead) at the onset of recovery (30 d after FNX). Microglial cells visualized by f CD11b or g IBA-1 immunohistochemistry (magenta) that are nucleated (DAPI, blue) are indicated (open arrowhead). Scale bars, 30 μm (overview) and 10 μm (higher magnification of boxed area)

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