Fig. 2

Histological characterization of the patient cohort. a-f, outlier box plot for the distribution of necrosis, HIF-1α in vital, central or perinecrotic tumor areas, phosphorylated (P-)RPS6, P-PRAS40 and Iba1 in samples as indicated (horizontal line within the box is the median sample value; confidence diamond contains the mean and the upper and lower 95% of the mean; ends of the box represent the 25th and 75th quantiles; bracket outside of the box is the shortest half, which is the most dense 50% of observations). g-k, correlations of histological markers as indicated in a bivariate plot with a linear regression analysis. P and r² values as indicated. l one way analysis with outlier box plot of P-PRAS40 and P-RPS6 in EGFR amplified vs. non-amplified tumor specimens. P-value calculated using Student’s t-test