Fig. 6From: CNS-border associated macrophages respond to acute ischemic stroke attracting granulocytes and promoting vascular leakageEffects of BAM depletion on leukocyte infiltration after brain ischemia. Rats received clodronate (CL) or vehicle (V) liposomes in the left ventricle 4 days before occlusion of the right middle cerebral artery and the brain was analyzed 1 day after ischemia. a) mRNA was extracted from the contralateral (control) and ipsilateral (ischemic) brain hemispheres of rats treated with V (n = 7) or CL (n = 6) for qRT-PCR analysis. Values are expressed as fold versus the mean value in the control V group. CL does not reduce leukocyte chemoattractants that were upregulated by ischemia in CD163+ macrophages (see Fig. 3), such as Spp1, C1qbp, Il6, and Ccl17. b) Flow cytometry analysis of the brain hemisphere of control rats (n = 11 vehicle, V; n = 9 CL) and ischemic rats (n = 12 V; n = 11 CL) illustrates leukocyte infiltration in the ischemic group. c) Depletion of CD163+ macrophages (CL group) reduces the percentage of REA535+ granulocytes in the infiltrating myeloid cell population (CD45hiCD11b+) (*p = 0.039)Back to article page