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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: CNS-border associated macrophages respond to acute ischemic stroke attracting granulocytes and promoting vascular leakage

Fig. 6

Effects of BAM depletion on leukocyte infiltration after brain ischemia. Rats received clodronate (CL) or vehicle (V) liposomes in the left ventricle 4 days before occlusion of the right middle cerebral artery and the brain was analyzed 1 day after ischemia. a) mRNA was extracted from the contralateral (control) and ipsilateral (ischemic) brain hemispheres of rats treated with V (n = 7) or CL (n = 6) for qRT-PCR analysis. Values are expressed as fold versus the mean value in the control V group. CL does not reduce leukocyte chemoattractants that were upregulated by ischemia in CD163+ macrophages (see Fig. 3), such as Spp1, C1qbp, Il6, and Ccl17. b) Flow cytometry analysis of the brain hemisphere of control rats (n = 11 vehicle, V; n = 9 CL) and ischemic rats (n = 12 V; n = 11 CL) illustrates leukocyte infiltration in the ischemic group. c) Depletion of CD163+ macrophages (CL group) reduces the percentage of REA535+ granulocytes in the infiltrating myeloid cell population (CD45hiCD11b+) (*p = 0.039)

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