Fig. 4

Treatment with clodronate liposomes depleted BAMs in control and ischemic rats. a) Flow cytometry plots of CD11b⁺CD163⁺ cells in the brain of control rats treated i.c.v. with either vehicle liposomes (V) or clodronate liposomes (CL) 5 days before the analysis. b) CL reduced the numbers of brain CD11b⁺CD163⁺ cells (*p < 0.05, n = 4 per group). c) Rats received CL or V in the left ventricle 4 days before occlusion of the right MCA and the brain was analyzed by flow cytometry 1 day after ischemia. d) Flow cytometry analysis of CD11b⁺ CD163⁺ cells of the ischemic hemisphere (n = 12 V; n = 11 CL) and the corresponding hemisphere of control rats (n = 11 V; n = 9 CL) and shows reduction after treatment with CL versus V in both groups. *** p < 0.001, two-way ANOVA by treatment and condition. e) Immunohistochemistry and cell counting in brain shows reduced numbers of CD163⁺ cells in the contralateral and ipsilateral (infarcted core) cortical and subcortical regions of the CL group (n = 12) compared to the V group (n = 10). *** p < 0.001, ** p < 0.01. Scale bar: 20 μm