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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Membrane binding, internalization, and sorting of alpha-synuclein in the cell

Fig. 6

The A30P and A11P/V70P aSyn mutants are less internalized than WT aSyn. a ICC and b Immunoblotting of cells transfected with Rab4A–GFP and treated as in experiments shown in Fig. 5. d and e ICC and Immunoblotting of cells transfected with Rab5A-GFP and treated as above. g and h ICC and Immunoblotting of cells transfected with Rab5ACA-GFP (constitutively active) and treated as above. c, f and i Quantifications of the immunoblots in panels b, e and h. Dotted bars refer to the band corresponding to aSyn dimers (aSyn**), and clear bars refer to aSyn monomers (aSyn*). Statistical tests were performed using one-way ANOVA with repeated-measures for grouped analysis, followed by Tukey’s post-hoc tests. Data are expressed as mean ± SEM and a 0.5% general significance level was defined, with significance levels as follows: *: p < 0.05; **: p < 0.01; ***: p < 0.001. Statistical significance is indicated with the symbol “#” for the monomers, “+” for the dimers, and “*” for the combination between monomers and dimers. Scale bar: 30 μm

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