Fig. 6

Ppt1 deficient (Ppt1^−/−) neuronal cultures exhibit altered culture composition and impaired survival. a To analyse neuronal cell death and visualise neuronal morphology, WT and Ppt1^−/−neuronal cultures were stained for Map2 and CC3. Pictures of 14 day old cultures stained with nuclear marker DAPI (blue), showing more Map2 positive cells (green) in Ppt1^−/−neuronal cultures labelled with CC3 (red) that in WT cultures. Arrows indicate CC3/ DAPI positive cells in WT cultures and Map2/CC3/DAPI positive cells in Ppt1^−/− neuronal cultures. Scale bar = 50 μm. b Significantly fewer neurons were positively stained for parvalbumin/calretinin/calbindin in Ppt1^−/− neuronal cultures, indicating that fewer inhibitory neurons are present than in WT neuronal cultures after 7 days in vitro. c The percentage of CC3 positive cells did not differ between WT and Ppt1^−/−neuronal cultures after 9DIV, but was significantly higher in Ppt1^−/−cultures after 14DIV. d Ppt1^−/−neurons stained for Map2 had significantly smaller soma sizes after 7, 9 and 14 days in vitro (DIV) compared to WT neurons. (Data shown as Mean ± SEM using a one way ANOVA, n = 3)