Fig. 3

Impaired Survival of Ppt1 deficient (Ppt1^−/−) astrocytes. A lower cell number was observed in Ppt1^−/− astrocyte cultures than in wild type (WT) cultures, due to decreased cell survival. a Under basal and stimulated conditions the number of Ppt1^−/−astrocytes per coverslip was markedly lower than in WT cultures as indicated by significantly reduced counts of cells stained with the nuclear marker DAPI. b At 24, 48 and 72 h following seeding onto coverslips cell death was significantly higher in Ppt1^−/− astrocyte cultures than in their WT counterparts. c A live/dead cell death marker (red) revealed the increased presence of dying cells in Ppt1^−/−astrocyte cultures stained for GFAP (green), compared to those derived from WT mice. (Data shown as Mean ± SEM using a one way ANOVA, n = 3). Scale bar = 50 μm