Fig. 1

Ppt1 deficient (Ppt1^−/−) astrocytes exhibit a reactive phenotype. A Wild type (WT) and Ppt1^−/− Astrocytes were stained for glial fibrillary associated protein (GFAP) to assess GFAP expression and astrocyte morphology under basal and stimulated (LPS/IFNγ) conditions after 24 and 48 h. Immunostaining for GFAP (red) revealed the morphology of Ppt1^−/− and WT astrocytes under basal and stimulated conditions, with Ppt1^−/− astrocytes exhibiting a large flattened morphology (white arrow) under basal conditions. B A higher percentage of Ppt1^−/− astrocytes stained positively for (GFAP) under basal conditions, with no significant upregulation following stimulation. C The soma size or spread of Ppt1^−/−astrocytes was much larger under basal conditions than in WT astrocytes, however was reduced following stimulation for 24 and 48 h. (Data shown as Mean ± SEM using a one way ANOVA, n = 3). Scale bar = 50 μm