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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: RNA binding proteins co-localize with small tau inclusions in tauopathy

Fig. 1

TIA1 preferentially colocalizes with phosphorylated tau that is not in NFTs. Immunohistochemical analysis of the stress granule nucleating protein TIA1 (red) shows colocalization with phosphorylated tau (CP13 antibody against pSer202, green) in rTg4510 (a), PS19 (b) and human AD tissues (c) with DAPI in blue. d Immunohistochemistry of TIA1 on rTg4510 with and without immunosorbtion using TIA1 peptide, as well as TIA1 staining of TIA1 knockout mouse tissue, also show that the TIA1 antibody used for this analysis is specific for TIA1 and does not have off-target staining. e Further analysis of TIA1-tau colocalization in rTg4510 tissues indicates that TIA1 primarily has cytoplasmic colocalization with smaller tau aggregates (white arrow) over larger NFT-like tau aggregates (pink arrow). This is quantified in (f) using Imaris Bitplane software, where cytoplasmic TIA1 intensity negatively correlates with the size of tau tangles in neurons (R = − 0.1617 with a two tailed p value of 0.0404). g TIA1 also colocalizes with oligomeric tau stained using the TOC1 antibody specific for oligomeric tau

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