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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: E90 subunit vaccine protects mice from Zika virus infection and microcephaly

Fig. 5

E90 vaccination of dams protects neonatal brains from ZIKV infection, cell death and survival. The brains of neonates born to vaccinated or unvaccinated mice were injected with ZIKV or medium as in Fig. 4. Coronal brain sections were stained with different antibodies as indicated. a. Relative signal intensity for ZIKV or activated caspase3 (Casp3). Mock + Veh: n = 11/4 (ZIKV), 11/4 (Casp3); ZIKV + Veh: n = 9/4 (ZIKV), 12/4 (Casp3); ZIKV + E90: n = 11/4 (ZIKV), 11/4 (Casp3). b. Quantification of NeuN+ cell numbers. Mock + Veh: n = 8/4, ZIKV + Veh: n = 8/4, ZIKV + E90: n = 9/4. c. Quantification of S100β+ cells. Mock + Veh: n = 11/4, ZIKV + Veh: n = 9/4, ZIKV + E90: n = 8/4. d. Quantification of Iba1+ cells. Mock + Veh: n = 11/4, ZIKV + Veh: n = 9/4, ZIKV + E90: n = 8/4. e. Improved survival rate of ZIKV-infected neonatal mice (P2) in the vaccinated group at 120 days post-vaccination. n = 12 per group, ****p < 0.0001, Log-rank (Mantel-Cox) test. Data (a-d) represent means ± SEM. Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. ns: not significant. n: # of slices/# of individual brains. Scale bar = 100 μm (a-d)

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