Fig. 3

E90 protects embryonic brains from ZIKV infection, dysregulation of NPC proliferation, and loss of neurons. Mouse dams were vaccinated and embryos were infected as in Fig. 2. Coronal brain sections were stained with different antibodies as indicated. a. Relative signal intensity by fluorescent antibodies against ZIKV or the activated form of caspase3 (Casp3). Mock + Veh: n = 6/3, ZIKV + Veh: n = 6/3, ZIKV + E90: n = 7/3. b. Quantification of P-H3⁺ cell numbers in the cortices. Mock + Veh: n = 10/4, ZIKV + Veh: n = 8/4, ZIKV + E90: n = 9/3. c. Quantification of Sox2⁺ and Tbr2⁺ cell density. Mock + Veh: n = 10/4 (Sox2⁺, Tbr2⁺); ZIKV + Veh: n = 7/3 (Sox2⁺, Tbr2⁺); ZIKV + E90: n = 9/3 (Sox2⁺, Tbr2⁺). d. Quantification of Tbr1⁺ cell density in cortices. Mock + Veh: n = 11/4, ZIKV + Veh: n = 7/4, ZIKV + E90: n = 8/3. e. Quantification of NeuN⁺ cell density. Mock + Veh: n = 12/4, ZIKV + Veh: n = 8/4, ZIKV + E90: n = 8/3. All data are means ± SEM. Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. ns: not significant. n: # of slices/ # of individual brains. Scale bar = 100 μm (a-e)