Fig. 2

The E90 vaccine protects embryonic brains from ZIKV-induced microcephaly. a. Comparisons of brain sizes and cortex thickness between mock-infected mice (Mock + Veh), unvaccinated (ZIKV + Veh) and vaccinated (ZIKV + E90) infected groups. Embryonic brains were injected with ZIKV or medium at E13.5 and inspected at E18.5 as shown in Fig. 1d. Nissl staining of coronal sections is shown in the lower panel. Right panel shows measured cortex thickness. Mock + Veh: n = 12/4, ZIKV + Veh: n = 9/4, ZIKV + E90: n = 9/3. b. Nissl staining of different cortical layers. Right panel shows measured thickness of each layer. Mock + Veh: n = 12/4, ZIKV + Veh: n = 9/4, ZIKV + E90: n = 9/3. CP: cortical plate, SP: subplate, IZ: intermediate zone, SVZ: subventricular zone, VZ: ventricular zone. All data are means ± SEM. Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001; ^##p < 0.01, ^###p < 0.001. ns: not significant. n: # of slices/# of individual brains. Scale bar = 1 mm (a), 100 μm (b)