Fig. 8
From: Optic nerve as a source of activated retinal microglia post-injury
Full thickness ONT limited the magnitude of the retinal myeloid cell response in comparison to a partial nerve transection. The ophthalmic artery was spared in all transections. Retinas and optic nerve were harvested from CX3CR1YFP-creER:CD11cGFP mice at 11 days post-injury. The volume of retina was approximately 10-fold greater than optic nerve. The full length of the optic nerve was recovered for flow cytometric analysis. GFPhi and GFPlo microglia were gated on viable cells, doublet exclusion, expression of CD45medCD11bhi and exclusion of Ly6G+ cells. GFPhi and GFPlo subsets were limited to CX3CR1+ cells. Mean ± SD. NS, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001. Statistical analysis by one-way ANOVA with Tukey HSD post-test. 7–12 mice/group