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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Optic nerve as a source of activated retinal microglia post-injury

Fig. 7

Naive optic nerve of the CX3CR1YFP-creER:CD11cGFP mouse was well-populated with microglia and generated a strong response to injury. a GFPhi microglia were numerous in naïve optic nerve (A), and GFPlo microglia densely populated the same naïve optic nerve as shown in (B). The Z-stack of flat mounted optic nerve shown in panels A and B was obtained by scanning through the optic nerve to find its center, and then stacking six 3 μm optical sections centered in the middle of the nerve. b The optic nerve at 31 days post-ONC remained densely populated. CD11cGFP reporter = green; DAPI = blue; CX3CR1YFP reporter = yellow. c Quantitation of retinal myeloid cells by flow cytometry of retina and optic nerve from naïve and day 10 post-ONC donors. An optic nerve crush led to increases in GFPhi and GFPlo microglia in retina and optic nerve. Cells were gated on viable CD45medCD11bhiLy6G cells with doublet exclusion. GFPhi and GFPlo cell counts were limited to CX3CR1-YFPhi cells. Mean ± SD. NS, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001. Statistical analysis by one-way ANOVA with Tukey HSD post-test. 6–11 mice/group

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