Fig. 4

Fluorescence imaging of the fundus and optic nerve demonstrated the topography of axon loss and response by GFP^hi cells. a GFP-fluorescence fundus imaging was done post-ONT to monitor the progression and location of GFP^hi cells in the retinas from the left eye of CD11c^GFP mice. Mouse A is an untreated control and shows the retinal orientation in all fundus photos (D-dorsal, V-ventral, N-nasal, T-temporal). Mouse D is 7 days post-ONC. Fundus photos of mouse B (5 and 9 days post-full ONT) and mouse C (17 and 28 days post-full ONT) show the progression of GFP^hi cells in retina. The response to a partial ONT is shown in the panels of mouse E and F. The panels are similarly labelled and dated for days post-ON injury. Only GFP^hi cells are visible. b Cross sections of the optic nerve post-injury in CD11c^GFP mice. The distribution of anti-SMI-31 staining for RGC axons late at 35 days post-partial ONT correlated with the extent of the transection (two-thirds cut) estimated from fundoscopy. Blue = DAPI; Green = CD11c^GFP; Red = SMI-31; Light blue = Isolectin B₄. Bottom panels show control and 10 days post-ONC. Blue = DAPI; Green = CD11c^GFP; Red = anti-CD11b