Fig. 10

Detection of Ki67⁺ cells in the optic nerve and retina. a Rare Ki67⁺ cells found in retinal flatmounts of CD11c^GFP mice at 6 days post-ONC. b Analysis of the optic nerve of B6 mice 7 days post-ONC revealed Ki67⁺ cells; some were also CD11b⁺. c Onset of retinal inflammation in the R161 spontaneous murine model of autoimmune uveitis in R161H x CD11c^GFP mice on the (B10.R3 x B6)_F1 background revealed extensive cell proliferation in retinal flatmounts prepared from eyes harvested at 111 days of age. (F) Many Ki67⁺ cells were found in the central retina near the optic nerve head, including some that co-stained with CD11b. (G) Smaller numbers of Ki67⁺ cells were found in the periphery of the opposite, inflamed retina. d Counts of Ki67⁺ cells in crushed ON. Sixteen micron thick sections were cut and stained. Non-parametric statistic done with Kruskal-Wallis H test analysis. *, p < 0.05; **, p < 0.01