Fig. 1

An optic nerve crush (ONC) injury stimulated appearance of GFP^hiYFP^hi myeloid cells in the retina of CX3CR1^YFP-creER:CD11c^GFP mice. a Time course of post-ONC loss of RGC comparing self-closing forceps with DSAEK forceps. Gray shaded area at top of graph represents the normal number of RGC/field ±1 SD. Area of field = 0.19 mm². (Red: DSAEK forceps; Blue: #N7 self-closing forceps.) b Fluorescence fundus photos detecting the expression of GFP from GFP^hi myeloid cells. The same retina at 2 days and 6 days post-ONC is shown. c Representative flow cytometry results of analyses of GFP^hi and GFP^lo populations of retinal myeloid cells (gated on viable, doublet-excluded CD45^medCD11b⁺Ly6G⁻ cells). d Time course of the appearance of GFP^hi myeloid cells and GFP^lo microglia in retina after an acute ONC injury. Average of 4–9 retinas per time point