Fig. 4

Radiation-associated DIPGs are molecularly distinct from primary DIPGs. a Plot of recurrent mutations in previously published datasets (adult GBM [n = 500] [7]; primary DIPG [n = 55] [44]) demonstrates that the distribution of driving mutations in radiation-associated DIPG is more similar to recurrent alterations in adult GBM than primary DIPG. b Contributions of established COSMIC mutational signatures were determined for radiation-associated DIPG samples as compared to all other primary cases sequenced through same sequencing platform (MI-ONCOSEQ). c-d Cases with previous radiation in this cohort (including case 1 and 3 and primary DIPG at autopsy) show higher mutations and fusions per exome (p = 0.0043 and p = 0.0135, respectively using Mann Whitney test)