Table 2 Tau antibodies used in this study
Name | Specificity | Epitope (a.a) | Source and dilution |
|---|---|---|---|
A0024 Tau | All tau isoforms | 243–441 (2N4R) | Dako, rp (WB 1:1000; IHC 1:500) |
TAU-5 | All tau isoforms | 210–241 (2N4R) | ThermoFisher, mm (WB 1:1000) |
Tau-1 | All tau isoforms | 189–207 (2N4R) | Merck, mm, clone PC1C6 (WB 1:2000) |
TP70 | All tau isoforms | 428–441 (2N4R) | IOP, KCL, rp (WB 1:500) |
Anti tau RD3 | 3R tau Isoforms | 267–282 (2N3R) | Merck, mm, clone 8E6 (WB 1:1000; IHC 1:500) |
Anti tau RD4 | 4R tau isoforms | 275–291 (2N4R) | Merck, mm, clone 1E1/A6 (WB 1:1000) |
Anti 4R-tau | 4R tau isoforms | NS | Cosmo bio co., rp (WB 1:2000; IHC 1:1000) |
Anti 0 N-tau | 0 N Tau isoforms | 39–50 (0N3R) | BioLegend, mm (WB 1:500) |
AT8 | Tau â„— S202/T205 | Tau â„— S202/T205 | Innogenetics, mm (WB 1:1000) |
PHF-1 | Tau â„— S396/S404 | Tau â„— S396/S404 | Gift from Peter Davies, mm (WB 1:500) |
PHF13 | Tau â„— S396 | Tau â„— S396 | Cell Signaling, mm (WB 1:1000) |
CP13 | Tau â„— S202 | Tau â„— S202 | Gift from Peter Davies, mm (IHC 1:200) |