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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Characterisation of tau in the human and rodent enteric nervous system under physiological conditions and in tauopathy

Fig. 7

Tau isoform profile and phosphorylation state in rat primary culture of ENS. a Lysates of rat primary ENS and CNS cultures were subjected to immunoblot analysis using the pan-Tau antibody A0024 and the isoform specific antibodies 3R and 4R. b Primary culture lysates were treated with (+) or without (−) lambda phosphatase before immunoblot analysis with the pan-Tau antibody A0024 and the isoform specific antibody against 3R-tau. PGP9.5 was used as a loading control. c Primary culture of rat ENS were treated (+) or not (−) with a cocktail of 3 phosphatase inhibitors including 1 μM okadaic acid, 1 μM ciclosporine A and 6.75 μM sanguinarine (Ppase inhibitors) for 1 h. Fifteen μg of cell lysates were subjected to immunoblot analysis using Tau-1, AT8 and PHF-1 antibodies. IB is for immunoblot. The results shown in (a), (b) and (c) are representative of 2, 4 and 3 independent experiments, respectively

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