Fig. 6

Tau expression and post-translational modifications in colonic biopsies from patients progressive supranuclear palsy, Parkinson’s disease and control subjects. a Biopsies lysates were subjected to immunoblot analysis using antibodies against total tau (TAU-5) and against 3R and 4R isoforms. An antibody against protein gene product (PGP) 9.5 was used as a loading control. For quantification, the optical densities of tau-immunoreactive bands were measured, normalized to the optical densities of PGP9.5 immunoreactive bands in the same samples and expressed as percentages of controls. b Biopsies lysates were subjected to immunoblot analysis using AT8, PHF-1 and TAU-5 antibodies. The optical densities of phospho-tau-immunoreactive bands were measured, normalized to the optical densities of TAU-5 immunoreactive bands in the same samples, expressed as percentages of controls. c Biopsies lysates were subjected to immunoblot analysis using antibodies Tau Asp421 and TP70. The optical densities of immunoreactive bands were measured, normalized to the optical densities of TP70 immunoreactive bands in the same samples, expressed as percentages of controls. Data correspond to mean ± SEM for 9 control samples (C), 10 from Parkinson’s disease (PD) patients and 5 from progressive supranuclear palsy (PSP). Immunoblot (IB)