Fig. 5

Poor susceptibility of tau to dephosphorylation in mature ENS. a Sigmoid colon biopsies lysates from 2 control subjects (#183, 3 first lanes and #208, 2 last lanes) were subjected to immunoblot analysis using TAU-5, AT8, PHF-1 and Tau-1 and antibodies. Lysates were treated with (+ for 1 h and ++ for 3 h) or without (−) lambda phosphatase before immunoblotting. The effectiveness of dephosphorylation was confirmed by phospho-ERK immunoblot (P-ERK immunoblot). b Antibodies against total tau (Tau antibody A0024) and tau phosphorylated at serine 202 (CP13) (both shown in red) were used to detect tau in the myenteric plexus of the proximal colon from htau mice. eGFP expression is shown in green, together with merged images including Hoechst 33,342 nuclear labelling (blue). Scale bars are 100 μm. N = 3. c. Brain and proximal colon homogenates from htau mice were treated with or without lambda phosphatase (+ or -) and immunoblotted with pan-Tau antibody A0024. A recombinant human tau ladder was included on each blot. White lines indicate rearrangement of lanes within the same blot for clarity. Data and images are representative of three independent experiments