Fig. 4

Distribution and localization of tau in human, htau and wild-type mouse myenteric plexus. a Total tau antibody A0024 and the isoform-specific antibodies against 3R and 4R-tau were used to detect tau in the myenteric plexus of a human colonic sample. An antibody specific to betaIII-tubulin was used to specifically label neurons. Scale bar is 200 μm (b) Total tau antibody A0024 was used to detect tau in the myenteric plexus of the duodenum, jejunum, ileum, proximal colon and distal colon of 2-month-old htau, wild-type (WT) and tau knockout (KO) mice. Merged images show tau (red), EGFP (green) and Hoechst 33342 labelling of nucleic acids (blue). Scale bar is 100 μm. Images are representative of three independent experiments