Table 4 Sample size and performed statistical analysis
From: Rac1 activation links tau hyperphosphorylation and Aβ dysmetabolism in Alzheimer’s disease
Figure Number | Experiment | Test | Sample size (n) | P value |
|---|---|---|---|---|
Fig. 1a | Rac1 AD human brain | Mann Whitney | 12, 24 | 0.028 |
Fig. 1b | Rac1 human AD plasma | Kruskal-Wallis Dunn’s test | 102, 47, 72, 42 | 0.0005 CTRL vs AD MMSE< 18 p = 0.0002; MCI vs AD MMSE< 18 p = 0.045; AD MMSE≥18 vs AD MMSE< 18p = 0.0051; |
Fig. 3b | SET/GluR1 | Two-tailed One sample t test | 10,10,10,6,6 | CTRL vs Rac1-WT p = 0.039 CTRL vs Rac1-L61F37A, p = 0.037 |
Fig. 3d | pT181/GAPDH | Two-tailed One sample t test | 9, 7, 7,3,3 | CTRL vs Rac1-WT p = 0.023; CTRL vs Rac1-L61F37A, p = 0.014 |
Fig. 3d | pT181/Tau5 | Two-tailed One sample t test | 9, 7, 7,3,3 | CTRL vs Rac1-L61F37A, p = 0.045; |
Fig. 5b | Hippocampus 6 weeks Rac1GTP/Rac1 | Student t test | 4, 4 | 0.044 |
Fig. 5c | Cortex 7 months Rac1/GAPDH | Student t test | 6, 6 | 0.005 |
Fig. 6a | PSD95/Tuj1 | One-way Anova Turkey’s MC | 8, 9, 8 | 0.0064 C57 + Veh vs 3xTgAD + Veh * 3xTgAD + Veh vs 3xTgAD + Rac1 * |
Fig. 6d | Spine density | One-way Anova Turkey’s MC | 4, 4, 4 | 0.0061 C57 + Veh vs 3xTgAD + Veh * 3xTgAD + Veh vs 3xTgAD + Rac1 ** |
Additional file 1: Figure S2A | Aβ toxicity | Two-tailed One sample t test | 4, 4, 4 | Aβ0.1 μM 0.0044 Aβ0.5 μM 0.0088 Aβ1μM 0.0414 |
Additional file 1: Figure S4C | 3 h OA | Two-Tailed paired t test | 6, 6 | 0.003 |
Additional file 1: Figure S4C | 6 h OA | Two-Tailed paired t test | 4, 4 | 0.038 |