Fig. 2
From: Rac1 activation links tau hyperphosphorylation and Aβ dysmetabolism in Alzheimer’s disease
Rac1 mutant peptides interfere with APP metabolism. a Primary cortical neurons treated between DIV11 and DIV14 with Rac1 mutant peptides. After 24 h, cells were stained to visualize of F-actin, APP and Aβ (4G8), and dendrites (MAP2). The increase in F-actin fluorescence was considered as a sign of Rac1 activation. These representative images were obtained from one of four independent experiments. Scale bar 30 μm. b Representative spectra of Aβ isoforms from SH-SY5Y cell lysates treated with TAT-Rac1 mutant proteins or vehicle (n = 2 experiments in triplicate). Peak intensity is expressed in μA (μAmpere). 3400 and 4500 are m/z reference values expressed in Dalton in the spectrum scale (m/z for Aβ 11–42pyr is 3325 Da, m/z for Aβ 1–42 is 4520 Da)