Table 2 Immunohistochemistry panel
From: Doublecortin-expressing cell types in temporal lobe epilepsy
Antibody | Product code, Supplier | Dilution (method) | Target Epitope |
|---|---|---|---|
DCX Ab1 | 4606, Cell Signaling Tech. Boston, US. *Used in [28] | 1:250 (IHC, IF) | Amino acid sequence 40–70 and 350–410 of human DCX |
DCX Ab2 | AB18723, Abcam, Cambridge, UK. | 1:4000 (IF) | AA 300 to the C-terminus of synthetic human DCX |
DCX Ab3 | SC-8066, Santa Cruz Biotech. Heidelberg, Germany. | 1:400 (IF) | C-terminus 365–402 of human DCX |
DCX Ab4 | AB2253, EMD Millipore, Watford UK. | 1:1000 (IHC, IF) | C-terminus 350–365 |
Nestin | AB22035, Abcam, Cambridge, UK. | 1:1000 (IHC, IF) | 150 aa recombinant fragment from human nestin conjugated to GST |
Nestin | AB105389, Abcam, Cambridge, UK. | 1:100 (IF) | Synthetic peptide corresponding to the C terminus of Human Nestin. |
Sox 2 | AB5603, EMD Millipore, Hertfordshire, UK. | 1:400 (IF) | KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2 |
GFAP-∂ | AB93251, Abcam, Cambridge, UK, | 1:4000 (IF) | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Mouse GFAP ∂ |
GFAP | Z0334, DAKO, Cambridgeshire, UK. | 1:1500 (IF) | GFAP |
NeuN | MAB377, EMD Millipore, Hertfordshire, UK. | 1:100 (IF) | Purified neuronal nuclei |
Iba1 | 019–19,741, WAKO, Osaka, Japan. | 1:6000 (IF) | Synthetic peptide corresponding to C-terminus of Iba1 |
CD68 | AB783, Abcam, Cambridge, UK. | 1:50 (IF) | Macrophages, microglia |
CD34 | IR632, DAKO, Cambridgeshire, UK. | 1:25 (IF) | Endothelial cells |
Olig 2 | AB9610, EMD Millipore Hertfordshire, UK | 1:200 (IF) | Recombinant mouse Olig-2 |
PDGFR-beta | AB32570, Abcam, Cambridge, UK. | 1:1000 (IF) | Synthetic peptide within Human PDGF Receptor beta aa 1050 to the C-terminus |
MCM2 | 610,700, BD biosciences, Oxford, UK. | 1:900 (IF) | Human BM28 aa. 725–888 |